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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
14/12/2012 |
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Data da última atualização: |
17/11/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES JUNIOR, F. L.; MELO, I. S. de; DIAS, A. C. F.; ANDREOTE, F. D. |
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Afiliação: |
FABIO LINO SOARES JÚNIOR, Universidade de Mogi das Cruzes; ITAMAR SOARES DE MELO, CNPMA; ARMANDO CAVALCANTE FRANCO DIAS, ESALQ-USP; FERNANDO DINI ANDREOTE, ESALQ-USP. |
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Título: |
Cellulolytic bacteria from soils in harsh environments. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, Oxford, v. 28, n. 5, p. 2195?2203, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: It is believed that the exposure of organisms to harsh climate conditions may select for differential enzymatic activities, making the surviving organisms a very promising source for bioprospecting. Soil bacteria play an important role in degradation of organic matter, which is mostly due to their ability to decompose cellulose-based materials. This work focuses on the isolation and identification of cellulolytic bacteria from soil found in two environments with stressful climate conditions (Antarctica and the Brazilian semi-arid caatinga). Cellulolytic bacteria were selected using enrichments at high and low temperatures (4 or 60C) in liquid media (trypic soy broth?TSB and minimum salt medium?MM) supplemented with cellulose (1%). Many of the isolates (119 out of 254?46.9%) displayed the ability to degrade carboxymethyl-cellulose, indicating the presence of endoglucolytic activity, while only a minority of these isolates (23 out of 254?9.1%) showed exoglucolytic activity (degradation of avicel). The obtained isolates revealed a preferential endoglucolytic activity according to the temperature of enrichments. Also, the identification of some isolates by partial sequencing of the 16S rRNA gene indicated that the Bacteroidetes (e.g., Pedobacter, Chryseobacterium and Flavobacterium) were the main phylum of cellulolytic bacteria isolated from soil in Antarctica; the Firmicutes (e.g., Bacillus) were more commonly isolated from samples from the caatinga; and Actinobacteria were found in both types of soil (e.g., Microbacterium and Arthrobacter). In conclusion, this work reports the isolation of bacteria able to degrade cellulosebased material from soil at very low or very high temperatures, a finding that should be further explored in the search for cellulolytic enzymes to be used in the bioenergy industry. MenosABSTRACT: It is believed that the exposure of organisms to harsh climate conditions may select for differential enzymatic activities, making the surviving organisms a very promising source for bioprospecting. Soil bacteria play an important role in degradation of organic matter, which is mostly due to their ability to decompose cellulose-based materials. This work focuses on the isolation and identification of cellulolytic bacteria from soil found in two environments with stressful climate conditions (Antarctica and the Brazilian semi-arid caatinga). Cellulolytic bacteria were selected using enrichments at high and low temperatures (4 or 60C) in liquid media (trypic soy broth?TSB and minimum salt medium?MM) supplemented with cellulose (1%). Many of the isolates (119 out of 254?46.9%) displayed the ability to degrade carboxymethyl-cellulose, indicating the presence of endoglucolytic activity, while only a minority of these isolates (23 out of 254?9.1%) showed exoglucolytic activity (degradation of avicel). The obtained isolates revealed a preferential endoglucolytic activity according to the temperature of enrichments. Also, the identification of some isolates by partial sequencing of the 16S rRNA gene indicated that the Bacteroidetes (e.g., Pedobacter, Chryseobacterium and Flavobacterium) were the main phylum of cellulolytic bacteria isolated from soil in Antarctica; the Firmicutes (e.g., Bacillus) were more commonly isolated from samples from the caatinga; and Actinobacteri... Mostrar Tudo |
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Palavras-Chave: |
Cellulases enzymes; Endoglucanase; Exoglucanase. |
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Thesagro: |
Bacteria; Caatinga; Celulose. |
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Thesaurus Nal: |
Antarctica; Cellulolytic microorganisms; Cellulose. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02597naa a2200265 a 4500
001 1942635
005 2014-11-17
008 2012 bl uuuu u00u1 u #d
100 1 $aSOARES JUNIOR, F. L.
245 $aCellulolytic bacteria from soils in harsh environments.$h[electronic resource]
260 $c2012
520 $aABSTRACT: It is believed that the exposure of organisms to harsh climate conditions may select for differential enzymatic activities, making the surviving organisms a very promising source for bioprospecting. Soil bacteria play an important role in degradation of organic matter, which is mostly due to their ability to decompose cellulose-based materials. This work focuses on the isolation and identification of cellulolytic bacteria from soil found in two environments with stressful climate conditions (Antarctica and the Brazilian semi-arid caatinga). Cellulolytic bacteria were selected using enrichments at high and low temperatures (4 or 60C) in liquid media (trypic soy broth?TSB and minimum salt medium?MM) supplemented with cellulose (1%). Many of the isolates (119 out of 254?46.9%) displayed the ability to degrade carboxymethyl-cellulose, indicating the presence of endoglucolytic activity, while only a minority of these isolates (23 out of 254?9.1%) showed exoglucolytic activity (degradation of avicel). The obtained isolates revealed a preferential endoglucolytic activity according to the temperature of enrichments. Also, the identification of some isolates by partial sequencing of the 16S rRNA gene indicated that the Bacteroidetes (e.g., Pedobacter, Chryseobacterium and Flavobacterium) were the main phylum of cellulolytic bacteria isolated from soil in Antarctica; the Firmicutes (e.g., Bacillus) were more commonly isolated from samples from the caatinga; and Actinobacteria were found in both types of soil (e.g., Microbacterium and Arthrobacter). In conclusion, this work reports the isolation of bacteria able to degrade cellulosebased material from soil at very low or very high temperatures, a finding that should be further explored in the search for cellulolytic enzymes to be used in the bioenergy industry.
650 $aAntarctica
650 $aCellulolytic microorganisms
650 $aCellulose
650 $aBacteria
650 $aCaatinga
650 $aCelulose
653 $aCellulases enzymes
653 $aEndoglucanase
653 $aExoglucanase
700 1 $aMELO, I. S. de
700 1 $aDIAS, A. C. F.
700 1 $aANDREOTE, F. D.
773 $tWorld Journal of Microbiology & Biotechnology, Oxford$gv. 28, n. 5, p. 2195?2203, 2012.
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Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 55 | |
| 4. |  | ANDREOTE, F. D.; TEIXEIRA, M. A.; DIAS, A. C. F.; VIEIRA, R. F. Análise da diversidade de proteobacterias e actinobacterias em solos cultivados com morango sob diferentes práticas de manejo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR, UEL, 2008. 4p. CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 5. | | ANDREOTE, F. D.; TEIXEIRA, M. A.; DIAS, A. C. F.; VIEIRA, R. F. Análise da diversidade de proteobactérias e actinobactérias em solos cultivados com morango sob diferentes práticas de manejo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: resumos. Londrina: Embrapa Soja: SBCS: IAPAR, UEL, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | LACAVA, P. T.; ANDREOTE, F. D.; ARAÚJO, W. L.; AZEVEDO, J. L. Caracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGE. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 4, p. 637-642, abr. 2006 Título em inglês: Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | CANOVA, S. P.; DIAS, A. C. F.; HARAKAVA, R.; ANDREOTE, F. D.; MELO, I. S. de. Diversidade de actinobactérias isoladas da rizosfera de Rhizophora mangl e em manguezal preservado e impactado. In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 11.; SIMPÓSIO NACIONAL DE MICROBIOLOGIA DO SOLO, 10., 2008, Fortaleza. Livro de resumos. Fortaleza: Laboratório de Microbiologia Ambiental e do Pescado, 2008. p.1068-1070.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 10. | | CANOVA, S. P.; ANDREOTE, F. D.; DIAS, A. C. F.; HARAKAVA, R.; MELO, I. S. de. Diversity of actinobacteria in the rhizosphere of the typical mangrove tree Rhizophora mangle. In: SYMPOSIUM ON AQUATIC MICROBIAL ECOLOGY, 11., 2009, Piran, Slovenia. Abstract book. Piran: National Institute of Biology, 2009. 1 CD-ROM p. 202| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 16. | | AVILA, L. A.; ANDREOTE, F. D.; SANTOS, S. N.; KAVAMURA, V.; OLIVEIRA, V. M.; MELO, I. S. de. Adaptation versus competition in the composition of the community of Pseudomonas spp. in mangroves. In: INTERNATIONAL CONGRESS ON EXTREMOPHILES, 8., 2010, Ponta Delgada. Book of Abstracts... Ponta Delgada, 2010. 1 CD-ROM. p. 144. p. 245| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 17. | | AVILA, L. A.; ANDREOTE, F. D.; SANTOS, S. N.; KAVAMURA, V.; VALENTE, A. M. M. P.; MELO, I. S. de. Antimicrobial activity of strains of Pseudomonas sp. of Brazilian mangrove. In: INTERNATIONAL CONGRESS ON EXTREMOPHILES, 8., 2010, Ponta Delgada. Book of Abstracts... Ponta Delgada, 2010. 1 CD- ROM. P 145. p. 246| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 18. | | SANTOS, S. N.; KAVAMURA, V. N.; AVILA, L. A.; SILVA, J. L. da; ANDREOTE, F. D.; MELO, I. S. de. Antimicrobial and antioxidant activity of an endophytic fungus of Combretum leprosum, an endemic plant from a Brazilian semi-arid region. In: INTERNATIONAL CONGRESS ON EXTREMOPHILES, 8., 2010, Ponta Delgada. Book of Abstracts... Ponta Delgada, 2010. 1 CD-ROM. P 61. p. 162| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | DIAS, A. C. F.; ANDREOTE, F. D.; RIGONATO, J.; FIORE, M. F.; MELO, I. S. de; ARAUJO, W. L. The bacterial diversity in a Brazilian non-disturbed mangrove sediment. Antonie van Leeuwenhoek, v. 98, n. 4, p. 541-551, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 55 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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